Review



c314s plasmids  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Thermo Fisher c314s plasmids
    Cys-314 is important for ASBT S-acylation and transport function. A and B, cellular expression (A) and [3H]TC uptake (B) in HEK293 cells transfected with WT or <t>C314S</t> ASBT-V5. n = 3; ****, p < 0.0001 compared with WT. C, Western blotting of bound (S-acylated) and unbound (nonacylated) acyl-RAC fractions from HEK293 cells transfected with WT or C314S ASBT-V5 (representative of three Western blots with similar results). D, a representative Western blotting for cell surface biotinylation of WT and C314S ASBT-V5 fusion proteins.
    C314s Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c314s plasmids/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    c314s plasmids - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "S -acylation modulates the function of the apical sodium-dependent bile acid transporter in human cells"

    Article Title: S -acylation modulates the function of the apical sodium-dependent bile acid transporter in human cells

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA119.011032

    Cys-314 is important for ASBT S-acylation and transport function. A and B, cellular expression (A) and [3H]TC uptake (B) in HEK293 cells transfected with WT or C314S ASBT-V5. n = 3; ****, p < 0.0001 compared with WT. C, Western blotting of bound (S-acylated) and unbound (nonacylated) acyl-RAC fractions from HEK293 cells transfected with WT or C314S ASBT-V5 (representative of three Western blots with similar results). D, a representative Western blotting for cell surface biotinylation of WT and C314S ASBT-V5 fusion proteins.
    Figure Legend Snippet: Cys-314 is important for ASBT S-acylation and transport function. A and B, cellular expression (A) and [3H]TC uptake (B) in HEK293 cells transfected with WT or C314S ASBT-V5. n = 3; ****, p < 0.0001 compared with WT. C, Western blotting of bound (S-acylated) and unbound (nonacylated) acyl-RAC fractions from HEK293 cells transfected with WT or C314S ASBT-V5 (representative of three Western blots with similar results). D, a representative Western blotting for cell surface biotinylation of WT and C314S ASBT-V5 fusion proteins.

    Techniques Used: Expressing, Transfection, Western Blot



    Similar Products

    c314s plasmids  (Thermo Fisher)
    90
    Thermo Fisher c314s plasmids
    Cys-314 is important for ASBT S-acylation and transport function. A and B, cellular expression (A) and [3H]TC uptake (B) in HEK293 cells transfected with WT or <t>C314S</t> ASBT-V5. n = 3; ****, p < 0.0001 compared with WT. C, Western blotting of bound (S-acylated) and unbound (nonacylated) acyl-RAC fractions from HEK293 cells transfected with WT or C314S ASBT-V5 (representative of three Western blots with similar results). D, a representative Western blotting for cell surface biotinylation of WT and C314S ASBT-V5 fusion proteins.
    C314s Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c314s plasmids/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    c314s plasmids - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Cys-314 is important for ASBT S-acylation and transport function. A and B, cellular expression (A) and [3H]TC uptake (B) in HEK293 cells transfected with WT or C314S ASBT-V5. n = 3; ****, p < 0.0001 compared with WT. C, Western blotting of bound (S-acylated) and unbound (nonacylated) acyl-RAC fractions from HEK293 cells transfected with WT or C314S ASBT-V5 (representative of three Western blots with similar results). D, a representative Western blotting for cell surface biotinylation of WT and C314S ASBT-V5 fusion proteins.

    Journal: The Journal of Biological Chemistry

    Article Title: S -acylation modulates the function of the apical sodium-dependent bile acid transporter in human cells

    doi: 10.1074/jbc.RA119.011032

    Figure Lengend Snippet: Cys-314 is important for ASBT S-acylation and transport function. A and B, cellular expression (A) and [3H]TC uptake (B) in HEK293 cells transfected with WT or C314S ASBT-V5. n = 3; ****, p < 0.0001 compared with WT. C, Western blotting of bound (S-acylated) and unbound (nonacylated) acyl-RAC fractions from HEK293 cells transfected with WT or C314S ASBT-V5 (representative of three Western blots with similar results). D, a representative Western blotting for cell surface biotinylation of WT and C314S ASBT-V5 fusion proteins.

    Article Snippet: Cells were transfected with 1 μg/well or 12 μg/dish of ASBT-V5 WT or C314S plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.

    Techniques: Expressing, Transfection, Western Blot